MOESM4 of Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process

Additional file 4: Figure S4. Expression pattern of scFv antibody protein without and with different fusion tags at different biological conditions. All of the four clones, scFv-pET28a(+), His.scFv-pET28a(+), GST.scFv-pGEX-4T-1 and MBP.scFv-pMAL-p5X were expressed in (a) 1 mM IPTG concentration at 37 °C for 4 h, then (b) 0.5 mM IPTG concentration at 30 °C for 4 h and (c) in 0.5 mM IPTG concentration at 20 °C for overnight.