MOESM4 of EFTUD2 gene deficiency disrupts osteoblast maturation and inhibits chondrocyte differentiation via activation of the p53 signaling pathway

Additional file 4: Figure S3. Zebrafish with eftud2 gene knockdown showed aberrant cartilage development. A, B: Larvae treated with an eftud2 morpholino (EMO) at 3dpf exhibited disrupted formation of Meckel’s cartilage (a) and the ceratohyals (b) upon alcian blue and alizarin red staining compared with the WT fish, fish injected with a mismatch morpholino (EMIS-MO) and fish rescued with normal human EFTUD2 mRNA (Rescue). A shows the lateral view, and B shows the ventral view. C, D: Bone and cartilage staining among different groups of larvae (WT, EMO, rescue, EMIS-MO) also suggested abnormal cartilage development at 5dpf.c, the ethmoid bones.