MOESM4 of Development of a genome-editing CRISPR/Cas9 system in thermophilic fungal Myceliophthora species and its application to hyper-cellulase production strain engineering

Additional file 4: Figure S4. Verification of triple-gene deletions of cre-1, res-1, gh1-1 and alp-1 in selected transformants. (A) Schematic of homologous recombination (HR) of cre-1, res-1 and gh1-1 mediated by Cas9, sgRNAs and donor DNA. (B) PCR analysis of triple-gene deletion of cre-1, res-1 and gh1-1 in selected transformants with specific paired primers (cre1/gh1-1/res1-out-F and cre1/gh1-1/res1-in-R). The expected length of disrupted transformants was 1.9 kb, while that of the host strain (rightmost lane) was 1.0 kb.