MOESM4 of Circular RNA circERBB2 promotes gallbladder cancer progression by regulating PA2G4-dependent rDNA transcription
journal contributionposted on 22.11.2019 by Xince Huang, Ming He, Shuai Huang, Ruirong Lin, Ming Zhan, Dong Yang, Hui Shen, Sunwang Xu, Wei Cheng, Jianxiu Yu, Zilong Qiu, Jian Wang
Any type of content formally published in an academic journal, usually following a peer-review process.
Additional file 4: Figure S3. circERBB2 promotes growth of GBC cells in vitro. a qPCR showed that circERBB2, but not ERBB2 mRNA, was effectively silenced by two siRNAs targeting back-splicing sequence of circERBB2 (n = 3). b CCK8 assay showed that silencing of circERBB2 by siRNAs impaired proliferation of SGC-996 cells (n = 3). c CCK8 assay showed that silencing of circERBB2 by siRNAs impaired proliferation of GBC-SD cells (n = 3). d Silencing of circERBB2 by siRNAs impaired clone formation ability of GBC cells. e gDNA sequence of sgRNA-targeted region of SGC-996Alu−/− and GBC-SDAlu−/− cells. f Structure of pLenti-CMV-circERBB2 vector and relative abundance of circERBB2 in GBC cells with or without OE of circERBB2 (n = 3). Quantitative data from three independent experiments was presented as mean ± SD (error bars). P-values were determined by paired, two-tailed two sample t-test. *: p < 0.05; **:p < 0.01.