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MOESM4 of Circular RNA circERBB2 promotes gallbladder cancer progression by regulating PA2G4-dependent rDNA transcription

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posted on 22.11.2019 by Xince Huang, Ming He, Shuai Huang, Ruirong Lin, Ming Zhan, Dong Yang, Hui Shen, Sunwang Xu, Wei Cheng, Jianxiu Yu, Zilong Qiu, Jian Wang
Additional file 4: Figure S3. circERBB2 promotes growth of GBC cells in vitro. a qPCR showed that circERBB2, but not ERBB2 mRNA, was effectively silenced by two siRNAs targeting back-splicing sequence of circERBB2 (n = 3). b CCK8 assay showed that silencing of circERBB2 by siRNAs impaired proliferation of SGC-996 cells (n = 3). c CCK8 assay showed that silencing of circERBB2 by siRNAs impaired proliferation of GBC-SD cells (n = 3). d Silencing of circERBB2 by siRNAs impaired clone formation ability of GBC cells. e gDNA sequence of sgRNA-targeted region of SGC-996Alu−/− and GBC-SDAlu−/− cells. f Structure of pLenti-CMV-circERBB2 vector and relative abundance of circERBB2 in GBC cells with or without OE of circERBB2 (n = 3). Quantitative data from three independent experiments was presented as mean ± SD (error bars). P-values were determined by paired, two-tailed two sample t-test. *: p < 0.05; **:p < 0.01.

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National Science Foundation of China

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