12936_2018_2431_MOESM4_ESM.pdf (317.75 kB)
MOESM4 of Chimeric Plasmodium falciparum parasites expressing Plasmodium vivax circumsporozoite protein fail to produce salivary gland sporozoites
journal contribution
posted on 2018-08-09, 05:00 authored by Catherin Marin-Mogollon, Fiona Pul, Shinya Miyazaki, Takashi Imai, Jai Ramesar, Ahmed Salman, Beatrice Winkel, Ahmad Othman, Hans Kroeze, Severine Chevalley-Maurel, Arturo Reyes-Sandoval, Meta Roestenberg, Blandine Franke-Fayard, Chris Janse, Shahid KhanAdditional file 4. Growth of asexual blood stages and mCherry expression in blood stages. Growth of asexual blood stages of two chimeric P. falciparum parasite lines (pf-pvcsp(vk210) and pf-pvcsp(vk247), a P. falciparum line lacking expression of CSP (PfΔcsp) and P. falciparum wild type (WT) parasites. Parasites of the different cloned lines were cultured in semi-automated culture system for a period of 7 days. Cultures were initiated with a parasitaemia of 0.5%. Arrows indicate the dilution of the cultures with fresh red blood cells to have a final parasitaemia of 1%. B. mCherry-expressing blood stages of PfΔcsp parasites where the csp gene has been disrupted by insertion of an mCherry expression cassette (see Additional file 5 for details of the generation of PfΔcsp). Scale bar, 7 µm.