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MOESM4 of Chimeric Plasmodium falciparum parasites expressing Plasmodium vivax circumsporozoite protein fail to produce salivary gland sporozoites

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posted on 2018-08-09, 05:00 authored by Catherin Marin-Mogollon, Fiona Pul, Shinya Miyazaki, Takashi Imai, Jai Ramesar, Ahmed Salman, Beatrice Winkel, Ahmad Othman, Hans Kroeze, Severine Chevalley-Maurel, Arturo Reyes-Sandoval, Meta Roestenberg, Blandine Franke-Fayard, Chris Janse, Shahid Khan
Additional file 4. Growth of asexual blood stages and mCherry expression in blood stages. Growth of asexual blood stages of two chimeric P. falciparum parasite lines (pf-pvcsp(vk210) and pf-pvcsp(vk247), a P. falciparum line lacking expression of CSP (PfΔcsp) and P. falciparum wild type (WT) parasites. Parasites of the different cloned lines were cultured in semi-automated culture system for a period of 7 days. Cultures were initiated with a parasitaemia of 0.5%. Arrows indicate the dilution of the cultures with fresh red blood cells to have a final parasitaemia of 1%. B. mCherry-expressing blood stages of PfΔcsp parasites where the csp gene has been disrupted by insertion of an mCherry expression cassette (see Additional file 5 for details of the generation of PfΔcsp). Scale bar, 7 µm.

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Leids Universitair Medisch Centrum

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