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MOESM4 of CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus
figure
posted on 2018-01-29, 05:00 authored by Ran Peng, Ye Wang, Wan-wan Feng, Xin-jing Yue, Jiang-he Chen, Xiao-zhuang Hu, Zhi-feng Li, Duo-hong Sheng, You-ming Zhang, Yue-zhong LiAdditional file 4: Figure S4. The sequence and expression vector of sgRNA. The promoter of pilA gene (shown in orange color) from M. xanthus drives transcription of sgRNAs. The sgRNA fragment containing the 20-nt specific targeting sequence (green), the tracrRNA scaffold (blue) and the human U6 terminator (red).
