13007_2017_183_MOESM4_ESM.pdf (84.25 kB)
MOESM4 of Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas
journal contribution
posted on 2017-05-15, 05:00 authored by Xi Cheng, Gai Liu, Wenting Ke, Lijuan Zhao, Bo Lv, Xiaocui Ma, Nannan Xu, Xiaoling Xia, Xuan Deng, Chunlei Zheng, Kaiyao HuangAdditional file 4: Figure S2. Different version of insertion cassettes. The 238Â bp intron3 of RbcS2 was fused to the luciferase at the site of CTGGAG/GTGCTG. The insertion cassette with or without splicing donor sequence at either side of AphVII gene was inserted to the restriction site of ApaI in RbcS2 intron3.
