MOESM4 of Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas

Additional file 4: Figure S2. Different version of insertion cassettes. The 238 bp intron3 of RbcS2 was fused to the luciferase at the site of CTGGAG/GTGCTG. The insertion cassette with or without splicing donor sequence at either side of AphVII gene was inserted to the restriction site of ApaI in RbcS2 intron3.