MOESM4 of Application of a New Multiplexed Array for Rapid, Sensitive, Simultaneous and Quantitative Assessment of Spliced and Unspliced XBP1

Additional file 4: Figure S3. Pre-clinical models of TNBC show results corelating with individual analyte assessment when assessed by XBP1 ratio. XBP1s and XBP1u levels expressed as a ratio of XBP1s (pg/mg)/XBP1u (pg/mg) in MCF7, SKBR3 and MDA-MB-231 cell lines (A). XBP1s and XBP1u levels expressed as a ratio of XBP1s (pg/mg)/XBP1u (pg/mg) in MCF7 and MDA-MB-231 cells treated with vehicle (DMSO) or IRE1α RNase inhibitors 4μ8C (32 μM) or MKC-8866 (20 μM) for 48 h and assessed XBP1 biochip (B). XBP1s and XBP1u levels expressed as a ratio of XBP1s (pg/mg)/XBP1u (pg/mg) in MDA-MB-231 cells following 48 h treatment with XBP1 splicing inducing chemotherapeutic Paclitaxel (10 nM) and IRE1α RNase inhibition (C).