MOESM4 of Antioxidants inhibit cell senescence and preserve stemness of adipose tissue-derived stem cells by reducing ROS generation during long-term in vitro expansion

Additional file 4: Figure S4. Antioxidants promote mouse ADSC cell migration. After treatment with 10 μM GSH or melatonin or the combination of GSH and melatonin, mouse ADSCs cultured for passage 3 (P3), passage 6 (P6) and passage 9 (P9) were used for migration assay. (a) Migration of passaged ADSCs (×200 magnification; scale bar, 50 μm). (b) Quantification of the number of migrated cells (n = 5 per group). ADSCs adipose tissue-derived stem cells, GSH reduced glutathione. (TIF 5004 kb)