posted on 2018-05-11, 05:00authored byGeorg Basler, Mitchell Thompson, Danielle Tullman-Ercek, Jay Keasling
Additional file 4. Quantification of extracellular n-butanol using GC-FID. The spreadsheet contains the estimated fraction of viable cells after incubation in PBS containing 0.2 and 1% n-butanol, and the measurement values of quantified extracellular n-butanol in induced (ttgABC + ) and non-induced strains (ttgABC - ). The original readings for n-butanol and the internal standard isoprenol are shown, as well as the n-butanol measurements normalized by the standard, and the derived concentrations as volume per volume and milligrams per milliliter. The inlay shows the n-butanol standard curve, indicating that concentrations are in a linear measurement range.