MOESM3 of p66ShcA functions as a contextual promoter of breast cancer metastasis

Additional file 3: Figure S3. p66ShcA is phosphorylated on Ser36 in 4T1-537 breast cancer cells. (A) Immunoblot analysis of whole cell lysates isolated from 4T1-537 p66-CR (VC), p66-CR (WT) and p66-CR (S36A) breast cancer cells treated with PBS, 1 mM phenformin (2 h) or 20 μM sodium arsenite (4 h) using pSer-p66ShcA, ShcA- and Tubulin-specific antibodies. (B) DCFDA flow cytometric staining demonstrating ROS production in response to phenformin or sodium arsenite treatment.