MOESM3 of Weight loss reduces basal-like breast cancer through kinome reprogramming
2016-04-01T05:00:00Z (GMT) by
Additional file 3. Kinome profiling revealed significant regulation of pathways by HFD that were reversed with weight loss. A and b. Quantitative comparison of kinases in unaffected mammary tissues from mice using MIB/MS was conducted. Legend indicates three iTRAQ runs with 2–4 samples pooled per group per run. The graphs indicates quantitative changes in kinase activity as a ratio of mice fed 60 % (a) or 60–10 % (b) diet relative to mice fed 10 % diet group. Ratio <1 denotes decreased kinase activity and >1 increased kinase activity. Kinase families are indicated (AGC: Containing PKA, PKG, PKC families; CAMK: Calcium/calmodulin-dependent protein kinase; CK1: Casein kinase 1; CMGC: Containing CDK, MAPK, GSK3, CLK families; STE: Homologs of yeast Sterile 7, Sterile 11, Sterile 20 kinases; TK: Tyrosine kinase; TKL: Tyrosine kinase-like). c. Mean kinase activity is reported for mice fed 60 % diet (dark grey) or 60–10 % diet (light grey) compared to mice on 10 % diet group. Error bars are not indicated for clarity. Statistically significant comparisons are reported in Fig. 5d.