13036_2019_223_MOESM3_ESM.pdf (141.52 kB)
MOESM3 of SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding
journal contribution
posted on 2020-01-14, 05:16 authored by Qingxiu Liu, Wei Zhang, Siying Chen, Zhenjing Zhuang, Yi Zhang, Lingli Jiang, Jun LINAdditional file 3: Figure S2. Optimization (A) and analysis (B) of the diffusion distance by using streptavidin (SA) and biotinylated oligodeoxynucleotides (Bio-ODN) by double diffusion. Arrows: target-DNA binding complexes. Concentration of Bio-ODN: 5 μM. Targets concentration: 1.0 μg/μL. Diffusion time was 6 h at room temperature. Bovine serum albumin (BSA) as a negative control.
