MOESM3 of SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding

Additional file 3: Figure S2. Optimization (A) and analysis (B) of the diffusion distance by using streptavidin (SA) and biotinylated oligodeoxynucleotides (Bio-ODN) by double diffusion. Arrows: target-DNA binding complexes. Concentration of Bio-ODN: 5 μM. Targets concentration: 1.0 μg/μL. Diffusion time was 6 h at room temperature. Bovine serum albumin (BSA) as a negative control.