MOESM3 of Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880

Additional file 3: Figure S3. Proton nuclear magnetic resonance (1H-NMR) spectroscopy analysis of galactitol. A total of 4 spectra are shown in Figure (all samples were dissolved in 90% H2O and 10% D2O). Panel (a) contains galactitol as the reference spectrum. Peaks at 3.84 ppm and 3.55 ppm were observed, noted with * symbol. Panel (b) shows the 1H spectrum of the culture media only. No galactitol signals were detected. Panel (c) contains the 1H spectrum of the product. The signals from galactitol show up clearly in this spectrum. Panel (d) is the spectrum collected after a few mg of galactitol powder were added directly to NMR tube (c). The signals from galactitol increased significantly, again indicating that the peaks in (c) are from galactitol.