MOESM3 of Probing the subcutaneous absorption of a PEGylated FUD peptide nanomedicine via in vivo fluorescence imaging

Additional file 3: Fig. S3. Fluorescence microscopy control experiments involving a combination of 20 kDa PEG-FUD-Cy5, human plasma fibronectin (FN), Alexa 488 Fluor labeled FN (A488-FN), and Hoechst nuclear stain treatments to AH1F human foreskin fibroblasts grown in a glass bottom dish. Each column indicates overlay of listed signal. The first, second, and third rows verify lack of overlap between the A488-FN, peptide-Cy5, and Hoechst signal. Scale bars = 50 Îźm.