MOESM3 of PTPRD-inactivation-induced CXCL8 promotes angiogenesis and metastasis in gastric cancer and is inhibited by metformin

Additional file 3: Figure S1. PTPRD silencing promotes cellular proliferation and migragion/invasion. Figure S2. Loss of PTPRD induce upregulation of CXCL8. Figure S3. HUVECs were cultured in supplement-lacking media with or without 1 ng/ml human recombinant CXCL8 (rhCXCL8). Figure S4. (A) MKN74 cells were treated with con-siRNA or CXCL8 siRNA for 48 h. Figure S5. PTPRD promoter methylation was assessed in various gastric cancer cell lines using methylation-specific PCR assay. Figure S6. The protein and mRNA expression of PTPRD were assessed upon treatment with metformin by western blot and RT/qRT-PCR using KATOIII, GCIY, and SNU668 whose PTPRD expression was relatively spared.