MOESM3 of PSMD1 and PSMD2 regulate HepG2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism

Additional file 3: Fig. S2. PSMD1 and PSMD2 expression level regulates the number and size of cellular lipid droplets in Huh7 cells. The Huh7 cells were seeded on the slide in a 24-well plate. Then, the cells were transfected with PSMD1/PSMD2 or NC siRNAs for 48 h for knockdown (A–C), or transfected with PSMD1/PSMD2 expression vector or NC vector for 48 h for overexpression (D–F). Subsequently, the cells were treated with 200 μM oleic acid for another 6 h. Then, the cells were fixed and stained by BODIPY493/503 and DAPI for observation by microscope. (B, E) The number of cellular LDs from different groups of cells. ImageJ software was used for the analysis. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 20; *p < 0.05) vs. NC. (C, F) The size of cellular LDs of different groups of cells. ImageJ software was used for the analysis. The statistical significance of differences between means was assessed using an unpaired Student’s t-test (n = 10; *p < 0.05;) vs. NC.