MOESM3 of Morphological, biochemical, transcriptional and epigenetic responses to fasting and refeeding in intestine of Xenopus laevis

Additional file 3: Figure S2. Epigenetic modifications on fabp1, fabp2, cdx2 and fxr genes in the intestines of fed, fasted and refed X. laevis. Chromatin samples were prepared from the intestines from animals that were fed for 22 days (fed), fasted for 22 days (fasted), and fasted for 21 days and then refed for 1 day (refed). Signals of ChIP on fabp1 (A, E, I, M, Q and U), fabp2 (B, F, J, N, R and V), cdx2 (C, G K, O, S and W), fxr (D, H, L, P, T and X) genes were detected by qPCR following immunoprecipitation with antibodies against H3K9me1 (A-D), H3K9me2 (E-H), H3K9me3 (I-L), H3K4me2 (M-P), H3K4me3 (Q-T), and normal IgG (U-X). Primers used in qPCR are shown in Additional file 5: Table S2. Each value is the mean ± SEM (n = 8). Distinct letters denote significantly different means, and were determined by one-way analysis of variance and Fisher’s least significant difference test for multiple comparisons (p < 0.05).