MOESM3 of MFAP5 promotes basal-like breast cancer progression by activating the EMT program
2019-03-07T05:00:00Z (GMT) by
Additional file 3: Figure S2. The inhibitory effect ofSB431542 and ly-411575 on TGF-β and Notch signaling pathways at different concentrations. BT20-LV-vehicle and BT20-LV-MFAP5 cells were treated with SB431542 or ly-411575 as indicated for 6 and 24 h, then the cells were collected to detect the expression of p-Smad2/Smad3, Hes1 and Hes5. (A) Immunofluorescence showed that SB431542 dose-dependently decreased the level of p-Smad2/Smad3 which was elevated by MFAP5. And at the concentration of 10 μM, SB431542 exhibited the largest inhibitory effect. (B) ly-411575 dose-dependently decreased the level of Hes1 and Hes5 induced by MFAP5 and the inhibitory effect was similar and more effective at 0.1 and 1 μM. **P < 0.01 vs corresponding LV-vehicle; ##P < 0.01 vs corresponding LV-MFAP5.