MOESM3 of KIF5B modulates central spindle organization in late-stage cytokinesis in chondrocytes

Additional file 3: Figure S3. Defects in the growth plate of Col2cre; Kif5bfl/− mice. (A) Immunostaining of KIF5B on the sections of the proximal tibial growth plate of P1 newborns. KIF5B is absent in the majority of the growth plate chondrocytes (indicated area). Scale bar: 150 μm. (B) RT-PCR analysis of total RNA from mainly the proliferating zones of the growth plates (C: Kif5bfl/+; M: Col2cre; Kif5bfl/−) showing the absence of Kif5a, Kif5b and Kif5c in mutant. RT-PCR of total RNA from wild-type mouse brain served as a positive control for amplification of Kif5a and Kif5c. (C) BrdU-incorporation analysis on the sections of the proximal tibial growth plate of P1 newborns. Scale bar: 150 μm.