MOESM3 of Intraepithelial neutrophils in mammary, urinary and gall bladder infections

Additional file 3. Bacterial communities are associated with intracellular neutrophils in gall bladder mucosal epithelial cells. Fluorescence channels comprising Figure 4B. Female C57BL/6 mice were challenged by injection of 105 CFUs of Salmonella enterica serovar Typhimurium/PoxyS-gfp strain SL1344 into the gall bladder which was harvested 24 hours after infection. Whole mounts of gall bladder were stained with DAPI (C, D) and phalloidin-TRITC (B and D). Confocal laser microscopy demonstrates GFP-expressing bacteria in gall bladder epithelial cells (yellow arrows in A and C, D) and intraepithelial neutrophils (white arrows in B–D). Composite image is presented in D. Original magnification X63 (A, B).