MOESM3 of Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803
2016-04-18T05:00:00Z (GMT) by
Additional file 3. Map of the autonomous vector pVZ325a , which was used to express the codon-adapted cDNA of the ispS gene from Pueraria montana for the expression in Synechocystis sp. PCC 6803. The synthetic P psaA* -ispS-oop DNA fragment contained upstream a SalI restriction site and downstream a PstI site, which facilitated subsequent insertion into the shuttle vector pVZ325.