MOESM3 of Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803

Additional file 3. Map of the autonomous vector pVZ325a [57], which was used to express the codon-adapted cDNA of the ispS gene from Pueraria montana for the expression in Synechocystis sp. PCC 6803. The synthetic P psaA* -ispS-oop DNA fragment contained upstream a SalI restriction site and downstream a PstI site, which facilitated subsequent insertion into the shuttle vector pVZ325.