13068_2016_503_MOESM3_ESM.docx (149.36 kB)
MOESM3 of Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803
journal contribution
posted on 2016-04-18, 05:00 authored by Nadin Pade, Sabrina Erdmann, Heike Enke, Frederik Dethloff, Ulf DĂźhring, Jens Georg, Juliane Wambutt, Joachim Kopka, Wolfgang Hess, Ralf Zimmermann, Dan Kramer, Martin HagemannAdditional file 3. Map of the autonomous vector pVZ325a [57], which was used to express the codon-adapted cDNA of the ispS gene from Pueraria montana for the expression in Synechocystis sp. PCC 6803. The synthetic P psaA* -ispS-oop DNA fragment contained upstream a SalI restriction site and downstream a PstI site, which facilitated subsequent insertion into the shuttle vector pVZ325.
