12967_2017_1360_MOESM3_ESM.pdf (1.36 MB)
MOESM3 of Gimatecan exerts potent antitumor activity against gastric cancer in vitro and in vivo via AKT and MAPK signaling pathways
journal contribution
posted on 2017-12-13, 05:00 authored by Zuhua Chen, Zhentao Liu, Wenwen Huang, Zhongwu Li, Jianling Zou, Jingyuan Wang, Xiaoting Lin, Beifang Li, Dongshao Chen, Yanting Hu, Jiafu Ji, Jing Gao, Lin ShenAdditional file 3: Figure S3. Gimatecan exerts antitumor activity via AKT and MAPK signaling pathways in HGC27 cells. (A) Gimatecan significantly inhibited the expression of TopI, pAKT, pMEK, and pERK, and activated the expression of p-p38 MAPK and pJUNK2 in HGC27 cells. Cells were starved in serum-free medium overnight, exposed to gimatecan or irinotecan for 48 h and harvested at 70–80% confluence. Total protein of HGC27 was extracted and the expression of TopI, pAKT, pMEK, pERK, p-p38 MAPK and pJNK2 were assessed by western-blotting followed by quantification and normalization by ImageJ. All data are means ± SD of three independent experiments. Compared with controls, *, p < 0.05.
