MOESM3 of Embryonic lethality is not sufficient to explain hourglass-like conservation of vertebrate embryos

Additional file 3: Figure S3. Windowing did not affect lethality in chicken embryos. Rate of lethal phenotypes of chicken embryos treated with TSA. Eggs were windowed and embryos were treated during the gastrula (Gst), pharyngula (Pha), and late (Lat) periods, or eggs were windowed but embryos not treated (Wnd). These are the same data shown in Fig. 1d. Additional data is the lethality of embryos that were not windowed or treated (Intct). Data are means; error bars are SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs Gst; †P < 0.05, ††P < 0.01, †††P < 0.001 vs Pha (Tukey–Kramer method). Lethality in Wnd (24.5%) was not significantly different from that of Intct (8.8%; P = 0.09, Tukey–Kramer method), suggesting that the effect of windowing on lethality could be ignored in this experiment.