MOESM3 of Differential transcriptomic profiling of filamentous fungus during solid-state and submerged fermentation and identification of an essential regulatory gene PoxMBF1 that directly regulated cellulase and xylanase gene expression

Additional file 3: Figure S1. Confirmatory analysis of 25 deletion mutants constructed from the parental strain ΔPoxKu70. (a) Schematic illustration of deletion mutant construction. (b) PCR analysis. M, 1 kb DNA markers; Lanes 1–3, three transformants for each candidate gene; Lane 4, ΔPoxKu70; Lane 5, ddH2O. The top panel shows amplification of the region using primers Gene-Left-F/G418VR; the middle panel shows amplification of the region using primers G418VF/Gene-Right-R; the bottom panel shows amplification of the region of the target gene using primers GeneVF/GeneVR. (c) Southern hybridisation analysis of deletion mutant ΔPOX08292. M, 1 kb DNA markers; Lane 1, ΔPoxKu70; Lane 2, ΔPOX08292-2; Lane 3, ΔPOX08292-6; Lane 4, ΔPOX08292-12.