40659_2016_95_MOESM1_ESM.docx (211.14 kB)
MOESM3 of Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence
journal contribution
posted on 2016-07-28, 05:00 authored by Shiva Marthandan, Uwe Menzel, Steffen Priebe, Marco Groth, Reinhard Guthke, Matthias Platzer, Peter Hemmerich, Christoph Kaether, Stephan DiekmannAdditional file 1: Figure S1. Induction or lack of apoptotic induction in primary human fibroblast strains subjected to 20 Gy irradiation. The blots show the lack of induction of two different apoptotic markers (Caspase-3 or BAX) in primary human fibroblast strains (HFF and MRC-5), 120 min after 20 Gy irradiation. Induction of either of the apoptotic markers in non-irradiated early PD fibroblasts were used as controls. As a positive control, the fibroblasts were subjected to induction of apoptosis by treatment with 2.0 μM Stauroporine for 96 h [35, 205–208].