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MOESM3 of Comparative proteome analysis in an Escherichia coli CyDisCo strain identifies stress responses related to protein production, oxidative stress and accumulation of misfolded protein

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posted on 2019-01-29, 05:00 authored by Isabel Guerrero Montero, Katarzyna Dolata, Rabea Schlüter, Gilles Malherbe, Susanne Sievers, Daniela Zühlke, Thomas Sura, Emma Dave, Katharina Riedel, Colin Robinson
Additional file 3: Figure S1. The swarming motilities of E. coli CyDisCo strains and P. aeruginosa. A swarming motility assay was performed using E. coli CyDisCo with an empty plasmid; expressing scFv; expressing misfolded scFv (mf_scFv) and P. aeruginosa PA01 as a positive control. Swarming plates (0.3% agar in LB medium) were spotted with 10 µL of overnight cultures and incubated for 24 h at 37 °C. After 24 h the E. coli CyDisCo strains did not show signs of cell swarming, while P. aeruginosa showed a positive swarming phenotype. Assays were performed in triplicate. Figure shows representative results from 24 h incubation.

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H2020 Marie Skłodowska-Curie Actions

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