12870_2019_2071_MOESM3_ESM.docx (79.34 kB)
MOESM3 of Clarification of the dispensability of PDX1.2 for Arabidopsis viability using CRISPR/Cas9
journal contribution
posted on 2019-11-05, 05:08 authored by Elisa Dell’Aglio, Ivan Dalvit, Sylvain Loubéry, Teresa FitzpatrickAdditional file 3: Table S2. Prediction of possible off-targets of the sgRNAs used in this study. Prediction of possible off-targets of the sgRNAs used in this study. The prediction was performed employing CRISPRdirect [20] of the Arabidopsis thaliana genome (version TAIR10). The search was performed with the PDX1.2 target sequences as listed. The number of sequences with a perfect match is shown and includes the on-target. In the case of CRISPRA the off-target hits are of AGI loci At1g55325 (involved in embryo patterning and cotyledon morphogenesis (Ito et al. 2011)) and At5g32690 (a pseudogene). (DOCX 79 kb)