MOESM3 of Characterization of Plasmodium relictum, a cosmopolitan agent of avian malaria

Additional file 3: Figure S3. Mature macrogametocytes (a–o) and microgametocytes (p–x) of the lineage pGRW4 of Plasmodium relictum in Hawaiian (a–j, p–v) and European (k–o, w, x) isolates during development in naturally infected Apapane Himatione sanguinea (a–f, p–s) and experimentally infected domestic canary Serinus canaria (g–j, k–o, t–x). Note that size and shape of mature gametocytes, number and position of pigment granules, morphology of parasite nuclei and influence of gametocytes on host cells are markedly variable and overlap in both isolates. Gametocytes of both isolates cannot be distinguished by morphological characters and patterns of their influence on host cells during their development in the same and different avian hosts. Furthermore, mature gametocytes of the lineage pGRW4 cannot be distinguished from mature gametocytes of the lineage pSGS1 (see Additional file 4: Figure S4). Long arrows—parasite nuclei. Short arrow—vacuole. Arrowheads—pigment granules. Triangle wide arrow—nucleolus. Giemsa-stained thin blood films. Scale bar = 10 μm.