MOESM3 of Carvacrol ameliorates acute campylobacteriosis in a clinical murine infection model

Additional file 3: Figure S3. Ileal apoptotic, proliferative and immune cell responses upon carvacrol treatment of C. jejuni infected mice. Starting 4 days prior peroral C. jejuni infection on days 0 and 1, secondary abiotic IL-10−/− mice were treated with synthetic carvacrol (CARVA; white boxes) or placebo (PLC; grey boxes) via the drinking water. The average numbers of (A) apoptotic (positive for caspase3, Casp3) and (B) proliferative/regenerative (positive for Ki67) ileal epithelial cells as well as of (C) T lymphocytes (positive for CD3) and (D) B lymphocytes (positive for B220) in the ileal mucosa and lamina propria from six high power fields (HPF, 400× magnification) per mouse were assessed microscopically in immunohistochemically stained small intestinal paraffin sections at day 6 post-infection. Naive mice served as uninfected controls. The total range, significance levels (p-values) determined by the Mann–Whitney U test and numbers of analyzed animals (in parentheses) are indicated. Data were pooled from four independent experiments.