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MOESM3 of Calcium ions in the aquatic environment drive planarians to food

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posted on 2019-11-07, 05:02 authored by Masato Mori, Maria Narahashi, Tetsutaro Hayashi, Miyuki Ishida, Nobuyoshi Kumagai, Yuki Sato, Reza Bagherzadeh, Kiyokazu Agata, Takeshi Inoue
Additional file 3: Figure S2. Motile cilia in the peripheral epithelium are not required for the feeding behavior. a. Kymograph of beating cilia of planarians during a 1 s period in Kanatani water in the absence of a particular ion. Asterisks denote successive ciliary beat cycles. Absence of calcium ions, potassium ions, or sodium ions did not affect ciliary beating. b. Removal of the cilia from the peripheral epithelium of planarians in Kanatani water supplemented with 1% ethanol (Cilia(−)), compared to Kanatani water alone (Ctrl). Many cilia (bracket) were observed on the peripheral epithelium of control planarians, but not on that of planarians treated with 1% ethanol. Scale bar: 10 μm. c. The food-localization assay of planarians with or without cilia. Planarians in tap water (Ctrl) showed a preference for moving to and staying in the region with the food, whereas cilia-removed planarians did not show such food-localization behavior. t = 600 s. d. Time spent in the target quadrant during assay of planarians with or without cilia. e. Speed of movement of planarians during the assay. f. The food-localization index of planarians with or without cilia.

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Japan Society for the Promotion of Science

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