MOESM3 of CHIP functions as an oncogene by promoting colorectal cancer metastasis via activation of MAPK and AKT signaling and suppression of E-cadherin

Additional file 3. Establishment of CHIP-sliencing and CHIP-overexpressing DLD-1 cells. (a) qRT-PCR analysis of the mRNA expression of CHIP between the two established siCHIP and sictrl cell lines. β-actin normalized gene expression, measured in triplicates was displayed. Significant differences were indicated (Student’s t-test, ***P < 0.001). (b) The protein expression of CHIP in the whole cell extract of siCHIP and sictrl cell lines was analyzed by western blotting. The level of each protein was normalized against Actin. (c) qRT-PCR analysis of the mRNA expression of CHIP between the two established hCHIP and ctrl cells. β-actin normalized gene expression, measured in triplicates is displayed. Significant differences were indicated (Student’s t-test, ***P < 0.001). (d) The protein expression of CHIP in the whole cell extract of hCHIP and ctrl cells was analyzed by western blotting. The level of each protein was normalized against Actin.