12951_2016_181_MOESM3_ESM.pdf (6.52 MB)
MOESM3 of Bacterial superglue enables easy development of efficient virus-like particle based vaccines
journal contribution
posted on 2016-04-27, 05:00 authored by Susan Thrane, Christoph Janitzek, Sungwa Matondo, Mafalda Resende, Tobias Gustavsson, Willem Jongh, Stine Clemmensen, Will Roeffen, Marga VegteâBolmer, Geert Gemert, Robert Sauerwein, John Schiller, Morten Nielsen, Thor Theander, Ali Salanti, Adam SanderAdditional file 3: Figure S2. Estimated coupling efficiencies of spy-VLP/antigen reactions. SDS-PAGE gels demonstrating that SpyTag or SpyCatcher fused antigens had reacted with corresponding spy-VLPs. (A) Survivin, (B) CIDR, (C) IL-5, (D) Pfs25, (E) Ag85A, (F) CSP, (G) HER2, (H) PCSK9, (I) CTLA-4, (J) PD-L1 and (K) VAR2CSA. Reactions were incubated for at least 12Â h at 4OC.