MOESM3 of A novel non-sulphamoylated 2-methoxyestradiol derivative causes detachment of breast cancer cells by rapid disassembly of focal adhesions

Additional file 3: Video S1. EE-15-one exposure results in the disassembly of the focal adhesions, severance of actin stress fibres connected to focal adhesions. For timelapse imaging, MCF-7 cells were transfected with LifeAct-mRFP (actin, red) and paxillin-GFP (focal adhesions, green). The following day cells were prepared for imaging every 30 s on a Zeiss LSM800 confocal microscope using a 63× magnification objective with environmental control. Cells were incubated with 5 µM EE-15-one. Scale bar, 20 µm.