MOESM3 of A closer look at Aspergillus: online monitoring via scattered light enables reproducible phenotyping

Additional file 3. Influence of shaking frequency variation and additives on the backscatter measurement with a round well plate. Complex YEPD medium was inoculated with 2 × 106 spores mL−1. Aspergillus giganteus was cultivated in a round well plate at 30 °C for 70 h. The biomass was analyzed non-invasively via scattered light measurement. The mean (thick line) and standard deviation (lighter area) of eight biological replicates are shown. Data for 850 rpm in the round well plate are shown in Fig. 3.