MOESM2 of gRNA validation for wheat genome editing with the CRISPR-Cas9 system

Additional file 2. Flow cytometry of protoplasts co-transformed with pUbi-Cas9-rbcS and pUbi-YFP-rbcS. Protoplasts were diluted to different concentrations (50,000–500,000 cells per 200 μL) for transformation, and either incubated with the DNA for 10 min prior to the addition of PEG or not incubated with the DNA (PEG added immediately to DNA/protoplast mixture). The percent of protoplasts expressing YFP is indicated. MFI, mean fluorescence intensity of YFP-expressing protoplasts.