MOESM2 of Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv

Additional file 1: Figure 2 Intact mass analysis and isoelectric point of recombinant M.tb GAPDH. MALDI-TOF confirms the presence of a single protein corresponding to a. MT-GAPDH-H and b. EC-H-GAPDH. Two dimensional gel analysis of rGAPDH purified from different source c. MT-rGAPDH d. MS-rGAPDH e. EC-rGAPDH where the gel was either stained with coomassie or western blot using rabbit α-GAPDH or mouse α-His. f. Peptide mass fingerprinting (PMF) of ~ 50 kD M. smegmatis protein that co-elutes with rPykA. Identified sequences are indicated in bold face.