12934_2016_537_MOESM2_ESM.tif (2.38 MB)
MOESM2 of Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv
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posted on 2016-08-11, 05:00 authored by Vishant Boradia, Pravinkumar Patil, Anushri Agnihotri, Ajay Kumar, Kalpesh Rajwadi, Ankit Sahu, Naveen Bhagath, Navdeep Sheokand, Manoj Kumar, Himanshu Malhotra, Rachita Patkar, Navi Hasan, Manoj Raje, Chaaya RajeAdditional file 1: Figure 2 Intact mass analysis and isoelectric point of recombinant M.tb GAPDH. MALDI-TOF confirms the presence of a single protein corresponding to a. MT-GAPDH-H and b. EC-H-GAPDH. Two dimensional gel analysis of rGAPDH purified from different source c. MT-rGAPDH d. MS-rGAPDH e. EC-rGAPDH where the gel was either stained with coomassie or western blot using rabbit α-GAPDH or mouse α-His. f. Peptide mass fingerprinting (PMF) of ~ 50 kD M. smegmatis protein that co-elutes with rPykA. Identified sequences are indicated in bold face.
