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MOESM2 of Vitamin C induces specific demethylation of H3K9me2 in mouse embryonic stem cells via Kdm3a/b

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posted on 2017-07-12, 05:00 authored by Kevin Ebata, Kathryn Mesh, Shichong Liu, Misha Bilenky, Alexander Fekete, Michael Acker, Martin Hirst, Benjamin Garcia, Miguel Ramalho-Santos
Additional file 2: Figure S2. Analysis of H3K9me2 in G9a and GLP knockout ES cells treated with vitamin C. A) Western blot for H3K9me2 in wild-type parental TT2, G9a knockout, and GLP knockout ES cells ± vitamin C. B) Immunofluorescence for H3K9me2 in GiP ES cells ± vitamin C and untreated wild-type TT2, G9a knockout, and GLP knockout ES cells. GiP ES cells treated with vitamin C show a H3K9me2 staining pattern that is similar to G9a and GLP knockout ES cells. Scale bar represents 20 μm.

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Canadian Cancer Society Research Institute

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