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MOESM2 of Targeting the orphan nuclear receptor NR2F6 in T cells primes tumors for immune checkpoint therapy

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posted on 2020-01-15, 05:11 authored by Victoria Klepsch, Maria Pommermayr, Dominik Humer, Natascha Brigo, Natascha Hermann-Kleiter, Gottfried Baier
Additional file 1: Figure S1 Characterization of Cas9 transgenic mice. (A) FACS analysis of GFP expression in CD4+ T cells, CD19+ B cells, Gr1+ granulocytes, and CD11b+ monocytes derived from the Cas9 transgenic mouse on a B6 background [33]. Cells were isolated from LN (first column), spleen (middle) or thymus/BM (third column). Cells from wildtype C57Bl/6 mice (grey) and Cas9 transgenic mice (pink) are shown in the same FACS plots. (B) Western blot analysis of lysates prepared from isolated CD3+ T cells of wildtype or Cas9 transgenic mice using a Flag antibody. (C) Representative histogram of GFP expression in wildtype, single transgenic Cas9, or double transgenic Cas9 mice.

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H2020 European Research Council

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