MOESM2 of Spectral tuning mediated by helix III in butterfly long wavelength-sensitive visual opsins revealed by heterologous action spectroscopy

Additional file 2: Figure S2. Secondary structure of PxRh3. Solid black circles indicate amino acid residues different from those of PxRh1. The third intracellular loop of PxRh3, which was replaced with that of Gs-coupled jellyfish opsin for the cAMP-based heterologous action spectroscopy, is boxed with a dotted line. Boundaries of helices for making chimeric mutants between PxRh1 and PxRh3 are indicated by black lines.