MOESM2 of Site-specific regulation of histone H1 phosphorylation in pluripotent cell differentiation

2017-05-22T05:00:00Z (GMT) by Ruiqi Liao Craig Mizzen
Additional file 2: Figure S1. Validations of custom H1 antibodies that have not been published previously. A, Recombinant H1 variants were analyzed by immunoblotting with our custom antisera for H1.5 and H1.0. B, PCA-extracted crude H1 from WI-38 VA-13 cells and a mixture of recombinant H1.5 and H1.0 were analyzed by immunoblotting with our custom antisera against pS18-H1.5. C, Antisera against pS18-H1.5 was mock-treated (none) or preadsorbed with pS18-H1.5 antigen peptide (pS18) or the corresponding non-phosphorylated peptide (S18) prior to immunoblotting with HeLa whole-cell lysate. D, HeLa and WI-38 VA-13 whole-cell lysates (WCL) and PCA-extracted crude H1 (PCAS) from WI-38 VA-13 cells were analyzed by immunoblotting with our custom antisera against H1.5 and H1.0.