MOESM2 of Silencing of one copy of the translation initiation factor eIFiso4G in Japanese plum (Prunus salicina) impacts susceptibility to Plum pox virus (PPV) and small RNA production

Additional file 2: Figure S2. Relative expression analysis of PpeIFiso4G10 (A), PpeIF4G (B) and PpeIF4E (C) in leaves of transgenic Japanese plum trees. Transcript levels were analyzed by qRT-PCR. All values were normalized to the TEFII reference gene and then compared to the wild type ‘Angeleno’ gene expression level. Error bars represent the standard deviation of two biological replicates each analyzed in triplicate. The standard deviation between replicates is indicated by vertical lines. Statistical analysis was performed using the Kruskal–Wallis rank sum test in R software v. 3.2.5. Transgenic and wild type Japanese plum lines labelled with the same letter are statistically identical (P value < 0.05). No significantly different values of PpeIF4G (B) and PpeIF4E (C) expression was evidenced by the Kruskal-Wallis test at P value ≤0.05. AG: transgenic ‘Angeleno’ plum lines; LA: transgenic ‘Larry Ann’ plum lines. The transgenic line numbers are corresponding to the ones depicted in Fig. 1b.