Springer Nature
Browse
12936_2018_2414_MOESM2_ESM.tif (1.34 MB)

MOESM2 of Self-assembling functional programmable protein array for studying protein–protein interactions in malaria parasites

Download (1.34 MB)
figure
posted on 2018-07-17, 05:00 authored by Gabriela Arévalo-Pinzón, María González-González, Carlos Suárez, Hernando Curtidor, Javier Carabias-Sánchez, Antonio Muro, Joshua LaBaer, Manuel Patarroyo, Manuel Fuentes
Additional file 2: Fig. S2. Protein subarray map. The localization of each construct and control in each sub-array is shown. Each sub-array contains 48 spots in total and each array contains 16 sub-arrays. Controls include printing with just clean buffer, BS3, BSA+BS3, cDNA without master mix, polyclonal anti-Halo antibody, anti-GST monoclonal antibody or anti-Halo monoclonal antibody. A sub-array of protein expression using the RRL system is shown below. Anti-Halo antibodies were printed to capture the Pv12 protein on the array.

Funding

Departamento Administrativo de Ciencia, Tecnología e Innovación

History