MOESM2 of Role of neuromedin B and its receptor in the innate immune responses against influenza A virus infection in vitro and in vivo

Additional file 2. Effect of NMB treatment on the expression of viral NP and cytokines in BMDMs. The BMDMs were mock-treated or treated with NMB after infection with PR8 (MOI = 1). NMB-treated cells were harvested at 16 hpi. The mRNA levels of NP, IFN-α, and IL-6 were measured by RT-PCR (A). qRT-PCR measurement of NP (B), IFN-α (C) and IL-6 mRNA expression (D). β-Actin was used as the reference housekeeping gene for internal standardization. ** P < 0.01.