Springer Nature
Browse
40643_2019_269_MOESM2_ESM.docx (769.04 kB)

MOESM2 of Optimized sampling protocol for mass spectrometry-based metabolomics in Streptomyces

Download (769.04 kB)
journal contribution
posted on 2019-09-11, 04:57 authored by Xiaoyun Liu, Tong Wang, Xiaojuan Sun, Zejian Wang, Xiwei Tian, Yingping Zhuang, Ju Chu
Additional file 2: Figure S1. In-house made rapid sampling device and its working procedure (A: diagrammatic sketch; B: front view; C: vertical view). Figure S2. Effect of different quenching solutions on the measured intracellular levels. Valine (Val), alpha-ketoglutarate (αKG) and glucose-6-phosphate (G6P) were exampled. Samples were taken from a culture at time = 60 h. AceB: acetone with base solution 5:1 (v/v) at − 30 °C; IsoaB: isoamylol with base solution 5:1 (v/v) at − 30 °C; ProB: propanol with base solution 5:1 (v/v) at − 30 °C; MethB: methanol with base solution 5:1 (v/v) at − 30 °C; Meth60: 60% methanol at − 30 °C, respectively. Base solution was made up of acetone: ethanol = 1:1 (mol/mol). Data were averages ± standard deviation of three replicate samples, each was analyzed in triplicate.

Funding

Major State Basic Research Development Program of China

History