MOESM2 of Intraepithelial neutrophils in mammary, urinary and gall bladder infections

Additional file 2. Bacterial communities are associated with intracellular neutrophils in urinary bladder transitional epithelial cells. Female C57BL/6 mice were challenged by intra-urethral inoculation with 107 CFUs human urinary pathogenic E. coli strain UTI89 and its bladder was harvested 24 h after infection. Whole mounts of urinary bladder were stained with phalloidin-TRITC (A), cytox orange (B), DAPI (C). Confocal laser microscopy demonstrates a large aggregation of intracellular bacterial community (IBC) in superficial umbrella bladder epithelial cell (yellow arrows in A, B) and intraepithelial neutrophil (white arrows in A and C). Composite image is presented in D. The xy image is on the plan indicated by the horizontal and vertical dashed lines shown in the xz and yz images, respectively. Scale bars 20 µm (A–D).