posted on 2019-09-19, 04:09authored byHadrien Peyret, James Brown, George Lomonossoff
Additional file 2. Sequences of the cloning fragments of the synthetic 5′ and 3′UTRs. Restriction sites (BsmB1 for 5′UTRs and Sap1 for 3′UTRs) are in bold and italicized. The sequence of the UTR once cloned into the expression cassette is underlined. Yellow: simplified synthetic CITE component complementary sequences. Green: synthetic CIRE component sequence. Blue: simplified BTE sequence. Magenta: CPMV RNA-2 3′UTR Y-loop structure.
Funding
Biotechnology and Biological Sciences Research Council