MOESM2 of Functional analysis of monoclonal antibodies against the Plasmodium falciparum PfEMP1-VarO adhesin
2016-01-15T05:00:00Z (GMT) by
Additional file 2: Table S1. Summary table of competition ELISAs. Competition ELISA were carried out as described in section “Methods”. In brief, saturating concentrations of unlabelled monoclonal D15-50, D15-68, E20-76, BD20E4, BDEE10, M21-17 and M21-30 IgG were incubated with eDBL1-coated plates for 2 h at 37 ℃, unbound IgG were washed out and biotinylated D15-50, E20-76 or BD20E4 IgG were added to individual wells at a concentration previously determined to generate a signal of approximately 1 OD after incubation at 4 ℃ for 20 min. Binding of the biotin-labelled IgG was monitored using streptavidin-labelled horseradish peroxidase.