MOESM2 of Defective angiogenesis in CXCL12 mutant mice impairs skeletal muscle regeneration

Additional file 2: Figure S1. (Related to Figure 1). Quantitative histological and cytometric parameters of the resting muscle from WT and CXCL12Gagtm/Gagtm mice. Quantification of (A) fibers number and (B) fibers diameter by Hematoxylin-eosin staining in the uninjured TA from WT (C57Bl6) and CXCL12Gagtm/Gagtm mice. Three animals (n=3) were used per condition and were repeated independently two times. (C) Quantification of Sirius Red positive/negative surface ratio. The mean ratio ± SEM (3 sections per mice, 10 images per sections) is given for the uninjured TA from KI (CXCL12Gagtm/Gagtm) mice. (D) Quantification of vessels number by CD31 immunostaining in the uninjured TA from WT (C57Bl6) and CXCL12Gagtm/Gagtm mice. Three animals (n=3) were used per condition and were repeated independently two times. (E) Quantification of GFP-positive cells by FACS analysis from both the TAs of WT (Flk1GFP/+) vs. KI (CXCL12Gagtm/Gagtm :: Flk1GFP/+) mice. (n=5 mice per condition). (F) Quantification of the sprouting vessels number in the resting muscle of WT (Flk1GFP/+) and KI (CXCL12Gagtm/Gagtm :: Flk1GFP/+) mice (n=5). Quantification of number of SCs by Pax7/GFP immunostaining (G) or by FACS analysis (H) in the uninjured TA from WT (Tg:Pax7nGFP) and CXCL12Gagtm/Gagtm mice (n=5 mice per condition). Data are given as the mean ± SEM. **** p < 0.0001.